Asst. Prof. Dr. Natharin Ngamwongsatit and Asst. Prof. Dr. Nathita Phumthanakorn, specialists from the faculty of veterinary science from Mahidol University, revealed the efficiency test result of Nano Care Essence Spray to restrain drug resistance in 15 pathogens species isolated from dogs and cats with lesions and infections on the skin or ears, including Methicillin-resistant S. aureus (MRSA) 5 species, Methicillin-resistant S. pseudointermedius (MRSP) 5 species, Multidrug-resistant (MDR) P. aeruginosa 5 species, S. aureus and P. aeruginosa 1 species, testing by 2 test methods that are the Agar well diffusion method which resulted that Nano Care Essence Spray capacity of 20, 40, and 60 microlitres can restrain all 15 pathogen species, and the Time kill assay methods which resulted that Nano Care Essence Spray can resist the growth of MRSA and MRSP in 2 – 12 hours and does not discover the growth of such pathogen in 24 hours  when compared to controlled substances PBS and MHB that the growth of the infection was observed at every test period, and it was discovered that it can reduce the growth of MDR P. aeruginosa in 2 – 4 hours and does not discover the growth of the pathogen in 8 – 24 hours when compared to the controlled substances PBS and MHB that the growth of the infection founded at every test period.  In conclusion, when compared to controlled substances, Nano Care Essence Spray effectively restrains the growth of MRSA, MRSP, and MDR P. aeruginosa up to 24 hours.